Research compound reference
NAD+
NAD+ is nicotinamide adenine dinucleotide: two nucleotides, one containing nicotinamide and one containing adenine, joined through their phosphate groups. The material is a white to off-white lyophilized powder for in vitro laboratory research. Purity by HPLC is reported per lot on a lot-specific Certificate of Analysis. This page is a technical reference to the material's identity, chemistry, analytical testing and laboratory handling.
For research use only. Not for human or veterinary, diagnostic or therapeutic use.
Structure identifiers
The identifiers a laboratory uses to confirm it is working with NAD+, as recorded in PubChem.1
Molecular formula
C21H27N7O14P2
Molecular weight
663.4 g/mol
Systematic name
[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2R,3S,4R,5R)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate
InChIKey
BAWFJGJZGIEFAR-NNYOXOHSSA-N
What is NAD+?
NAD+ is nicotinamide adenine dinucleotide: two nucleotides, one containing nicotinamide and one containing adenine, joined through their phosphate groups. It is catalogued under CAS 53-84-9 and PubChem CID 5892, with the molecular formula C21H27N7O14P2 and a molecular weight of 663.4 g/mol.1
The material described on this page is intended as a laboratory reagent.
Handling
NAD+ is a dry solid as received in the laboratory. A reference supplier lists storage of this compound as a solid at -20 °C.2
- Allow the sealed container to reach room temperature before opening, so moisture does not condense on the solid.
- Keep the solid dry, sealed and away from bright light.
- Avoid repeated freezing and thawing.
Storage and stability
| State | Temperature | Notes |
|---|---|---|
| Dry, sealed | -20 °C2 | Keep dry and away from bright light. |
| Freezing and thawing | Avoid repeated freezing and thawing | Applies to the dry solid. |
How each lot is tested
Each lot is tested by an independent third-party laboratory.3 The NAD+ certificate page names the testing laboratories and lists each lot's purity, net content and test date. Three measurements are reported.
Identity
Mass spectrometry
Checks that the measured molecular mass matches NAD+. The calculated monoisotopic mass is 663.11 Da.1
A matching mass is consistent with the expected composition. It does not by itself confirm the structure.
Purity
High-performance liquid chromatography (HPLC)
Separates the material from synthesis-related impurities. Purity is reported as the main peak's share of the total detected signal.
Reported per lot on the Certificate of Analysis.
Quantity
Net content
Measures the mass of material actually present in the sample tested.
Reported per lot on the Certificate of Analysis.
Technical notes
What does the Certificate of Analysis confirm?
Identity by mass spectrometry, purity by HPLC, and the net content of the sample.
Why is the material freeze-dried?
Dry solids are generally more stable than solutions. The material is lyophilized and kept sealed until use in the laboratory.
NAD+ lot certificates
Each listed NAD+ lot has its own certificate covering identity, purity and net content.
Catalogue entry: NAD+
More from the research reference
References
- National Center for Biotechnology Information. PubChem Compound Summary for CID 5892 (nicotinamide adenine dinucleotide). pubchem.ncbi.nlm.nih.gov/compound/5892. Accessed 20 October 2026.
- Sigma-Aldrich. β-Nicotinamide adenine dinucleotide hydrate, product N1511 (storage temperature -20 °C). sigmaaldrich.com. Accessed 20 October 2026.
- Oasis Labs. NAD+ Certificate of Analysis page, lot data and testing laboratories (certificate page), and testing methodology, myoasislabs.com/coa-process/.
References document the chemical identity, analytical characterisation and handling of this material.